vaginal epithelial cells  (Celprogen Inc)

Journal: Frontiers in Parasitology

Article Title: In vitro co-culture model of Trichomonas vaginalis , Candida albicans , and Lactobacillus crispatus : a system for assessing antimicrobial activity and microorganism interactions in vaginitis

doi: 10.3389/fpara.2025.1523113

Figure Lengend Snippet: Cytolysis measured by LDH release from human vaginal epithelial cells co-incubated with monocultures or co-cultures of Trichomonas vaginalis, Candida albicans and Lactobacillus crispatus. (A) Monocultures of L. crispatus (LC2, 5.53 x 10 6 CFU/mL) C. albicans (CA3, 3.33 x 10 4 CFU/mL), and T. vaginalis standard isolate (ATCC30236, 1.0 x 10 6 trophozoites/mL). Dual co-cultures: LC2 + CA3, ATC30236 + CA3, and ATCC30236 + LC2. Triple co-culture: ATCC30236 + CA3 + LC2. (B) Monocultures of L. crispatus (LC1, 5.53 x 10 7 CFU/mL), C albicans (CA3), and T. vaginalis fresh clinical isolate (TV-LACM6, 1.0 x 10 6 trophozoites/mL). Dual co-cultures: LC1 + CA3, TV-LACM6 + CA3, and TV-LACM6 + LC1. Triple co-culture: TV-LACM6 + CA3 + LC1. Positive control of cytolysis is HMVII cells treated with 0.2% Triton X-100. Results are expressed as a percentage of total cytolysis. Data are presented as the mean ± S.D. of at least two experiments. The percentage of LDH released from HMVII cells with the monocultures of T. vaginalis isolates was compared with that from HMVII cells with co-cultures with the protozoan. (*) indicates a significant difference.

Article Snippet: The cytolytic capacity of T. vaginalis can be measured by the release of the lactate dehydrogenase (LDH) enzyme from human vaginal epithelial cells (HMVII lineage). shows that co-incubation of the co-culture of ATCC T. vaginalis isolate (ATCC30236, at initial density 1.0 x 10 6 trophozoites/mL), C. albicans (CA3, 3.33 x 10 4 CFU/mL), and L. crispatus (LC2 5.53 x 10 6 CFU/mL) with vaginal cells led to a significant increase in the LDH release compared to the co-incubation of ATC30236 alone with vaginal cells.

Techniques: Incubation, Co-Culture Assay, Positive Control

Journal: RSC Advances

Article Title: Luliconazole–niacinamide lipid nanocarrier laden gel for enhanced treatment of vaginal candidiasis: in vitro , ex vivo , in silico and preclinical insights

doi: 10.1039/d4ra08397k

Figure Lengend Snippet: (A) In vitro antimicrobial activity of experimental lipid nanocarrier-embedded bigel (LZNMLNs2G2) via agar well diffusion method against Candida albicans vs. standard gel (Candid V gel) as positive control. DMSO was used as negative control. (B) Cytotoxicity analysis of nano-bigel (LZNMLNs2G2)/blank gel in selected vaginal epithelial cells.

Article Snippet: Primary Vaginal Epithelial Cells (ATCC PCS-480-010) were collected from American Type Culture Collection, Manassas, VA.

Techniques: In Vitro, Activity Assay, Diffusion-based Assay, Positive Control, Negative Control